COMPARATIVE ANATOMY OF OLFACTORY BULBS IN AFRICAN GIANT POUCHED RATS (Cricetomysgambianus, Waterhouse) AND WISTAR RATS.

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Date
2015-08
Authors
BUKAR, DAVID PINDAR
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Abstract
Comparative anatomy of olfactory bulbs of African Giant Rats (AGR) (Cricetomysgambianus,Waterhouse) and Wistar Rats (WR) was carried out using, twenty four adult (24) adult AGRs comprising twelve (12) females and males respectively, and twenty four (24) adult WRs comprising twelve (12) females and males respectively for this study. The AGRs and WRs were euthanized under light anaesthesia using gaseous chloroform in a confined glass container. After fixing the heads of the two rats in Bouins solution, the brain was carefully extracted, weighed and recorded. The olfactory bulb was then secured from the brain, weighed and recorded. Gross anatomical features of the brain and olfactory bulbs were examined from the dorsal, ventral and sagittal views taking into consideration their shapes, sizes, surfaces, and attachments. Furthermore, morphometric study of the brain and olfactory bulbs of the AGRs and WRs were conducted by measuring weight, length, height and the volume as morphometric parameters. The brain and olfactory bulbs were fixed in Bouin's solution, dehydrated in graded alcohol for 24 hours; the Bouin's solution was cleared using xylene for 2 hours. Routine paraffin embedding method for histological sectioning of the samples was used. Hematoxylin and Eosin, Cresyl Fast Violet and Enarson's staining methods were used toview and compare the individual cells types, cell layers and the cell organization of the olfactory bulbs of the female and male AGRs and WRs. Slides were viewed under light microscopy at various magnifications, while photomicrographs of olfactory bulbs and their individual cell types, cell layers and cell organization were taken using digital camera. This study has established a comparative base-line data for the brain and olfactory bulbs of both female and male AGRs and WRs giving a detailed description of the neuroanatomical characteristics with emphasis on the morphology, morphometry and histomorphology. The 3 stains gave a clear histology viii of the concentric laminar organization of the olfactory bulbs showing 6 layers in both rats.Glomerular cell layer in AGR was 2 layers thick, while that of WR was 3 layers thick. Mitral cells were numerous and well distributed in the mitral cell layer of both rats. Among the 3 stains,cresyl fast violet gave a better histograph of the olfactory bulbs layers and cells. There will be need to carry out further studies to determine and evaluate the macromastic features of the cell types, cell layers and cell organization using immunohistochemistry, neurone tracing method and micrometry
Description
A THESIS SUBMITTED TO THE SCHOOL OF POSTGRADUATE STUDIES, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE AWARD OF THE DEGREE OF MASTERS OF SCIENCE IN VETERINARY ANATOMY DEPARTMENT OF ANATOMY, AHMADU BELLO UNIVERSITY, ZARIA, NIGERIA
Keywords
COMPARATIVE,, ANATOMY,, OLFACTORY BULBS,, AFRICAN GIANT,, POUCHED,, RATS,, Cricetomysgambianus,,, Waterhouse,, WISTAR RATS,
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